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제품명 : #2082 - 5 nm Ni-NTA-Nanogold®

New!5 nm Ni-NTA-Nanogold®

Go straight to EM-
no silver or gold enhancement needed!Buy now

The new 5 nm Ni-NTA-Nanogold® provides new features, improved performance, and extends NTA-Ni(II) targeting technology to larger gold.

This probe has the same high resolution as the 1.8 nm Ni-NTA-Nanogold®, and the entire probe is still smaller than an IgG molecule, but the larger gold particles may be clearly visualized by standard TEM without silver or gold enhancement, even in wider views such as thick sections and whole cells. Blot sensitivity will be even higher due to the larger gold.

  • High visibility: The 5 nm gold particle is clearly visualized at TEM resolution without the need for silver or gold enhancement. This simplifies labeling and provides a more monodisperse size range. You may also use this probe for multiple labeling studies in conjunction with gold particles of different sizes, or with silver or gold-enhanced Nanogold.
  • Precise labeling resolution: the nitrilotriacetic acid - Ni(II) chelate is much smaller than an antibody or protein, and therefore when it is bound, the gold is much closer to its target: we estimate that the distance from the gold particle surface to the His tag is on the order of 1.5 nm. This makes NTA-Ni(II)-Nanogold® ideal for localizing sites in protein complexes or other macromolecular assemblies at molecular resolution.
  • High solubility and stability: 5 nm Ni-NTA-Gold is prepared using a modified gold particle, using a stable, highly hydrophilic surface functionalization.
  • Strong binding: binding constants for Ni(II)-NTA are very high due to the combination of the chelate effect of multiple histidine binding, and target binding of multiple Ni(II)-NTA functionalization. Dissociation constants are estimated to be between 10-7 to 10-13 M-1. For many applications, this provides binding strengths comparable to antibodies.
  • High penetration: 5 nm Ni-NTA-Gold is smaller than an unlabeled primary antibody, and can more easily penetrate into specimens and access sterically restricted interior sites, and perturbs the ultrastructure less. In some systems it may be used with stronger fixation or less permeabilization, enabling labeling with better ultrastructural preservation.
  • Super sensitivity: the larger gold particle provides higher sensitivity with virtually no background when used to detect His-tagged targets on blots. 10 ng of His-tagged ATF-1 was detected without silver or gold enhancement. Gold enhancement allows the detection of 0.5 ng, with no visible binding to an E Coli. extract control.
[Ni-NTA-5 nm Gold structure, and TEM View (141k)]
Top: Structure of NTA-Ni(II)-5 nm Nanogold®, showing the binding of the incorporated metal chelate to a His-tagged protein; distance from the gold particle surface to the His tag is estimated to be 1.5 nm. Above: Transmission electron micrograph of 5 nm NTA Nanogold: average diameter 5.11±0.84nm.

Instructions and technical information (html) 
[2080-PDF]  Instructions (PDF) 
[2080-PDF]  Material Safety Data Sheet (PDF)

See our 2005 paper on 5 nm Ni-NTA-Nanogold®.